A modified PF4-dependent, CD62p expression assay selectively detects serotonin-releasing antibodies in patients suspected of HIT
نویسندگان
چکیده
منابع مشابه
A modified ELISA that selectively detects monoclonal antibodies recognizing native antigen.
A modified ELISA procedure is described which permits selective detection of monoclonal antibodies reactive with the native antigen. The essence of the method is the use of immobilization methods that do not result in denaturation of the antigen during adsorption to the microtiter plates. This was accomplished by using polyclonal antibodies, adsorbed to the wells of the microtiter plate, as the...
متن کاملHeparin-dependent and -independent anti-platelet factor 4 autoantibodies in patients with systemic lupus erythematosus.
OBJECTIVE Antibodies that recognize complexes formed by platelet factor 4 (PF4) and heparin are involved in the pathogenesis of heparin-induced thrombocytopenia (HIT). This study was undertaken to investigate the prevalence and clinical correlations of anti-PF4 autoantibodies in patients with SLE. METHODS We studied 118 patients with SLE, 78 with primary immune thrombocytopenia (ITP), 27 with...
متن کاملWhat is the potential for overdiagnosis of heparin-induced thrombocytopenia?
Heparin-induced thrombocytopenia (HIT) is caused by platelet-activating antibodies that recognize platelet factor 4//heparin (PF4/H) complexes. According to the "iceberg model," only a subset of anti-PF4/heparin antibodies of IgG class evincing strong platelet-activating properties cause clinical HIT. Since many centers rely predominantly on an anti-PF4/polyanion enzyme-immunoassay (EIA) to dia...
متن کاملDistribution of Anti-leptospira Antibodies in the Sera of Patients Suspected Leptospirosis
Abstract Background and Objective: Leptospirosis is a zoonosis infectious disease that is prevalent in tropical and subtropical regions and is caused by the pathogenic serovars of leptospires. Hence, we aimed at investigating the prevalence of antibodies against these bacteria in the blood samples of suspected leptospirosis. Material and Methods: the human serum samples (N = 130) were ...
متن کاملPlasma exchange to remove HIT antibodies: dissociation between enzyme-immunoassay and platelet activation test reactivities.
Repeated therapeutic plasma exchange (TPE) has been advocated to remove heparin-induced thrombocytopenia (HIT) IgG antibodies before cardiac/vascular surgery in patients who have serologically-confirmed acute or subacute HIT; for this situation, a negative platelet activation assay (eg, platelet serotonin-release assay [SRA]) has been recommended as the target serological end point to permit sa...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Thrombosis and Haemostasis
سال: 2015
ISSN: 0340-6245,2567-689X
DOI: 10.1160/th15-02-0175